Markov model is one of the decision analysis models, which widely applied in pharmacoeconomic evaluation. The acquisition of transition probability parameters between different Markov health states is difficult in model building and they are usually can’t be obtained directly. In this study, we discussed the calculation of current Markov model transition probability parameters, especially for the time-dependent model. Furthermore, we performed a case analysis to explore how to calculate the transition probability and provide reference for researchers of this field