Objective: To evaluate the implementation of functional tasks of National Medical Centers and Regional Medical Centers of China from the perspective of medical staff. Methods: A total of 613 practitioners from 15 hospitals of the centers finished the setting approval in October, 2020 were surveyed. Descriptive analysis and rank-sum test were applied to explore the quantitative data. Results: According to medical staff, the implementation of centers' functional tasks in the aspects of medical care and disease prevention was fairly good, but the research and education aspects were not satisfactory. In terms of needs, "talent team construction" was focused. Conclusion: The centers are obliged to focus on the introduction and cultivation of high-level medical personnel. Collaborative mechanism of medical care, education, research, and utilisiation are needed be established and improved to meet clinical demands. The centers should strengthen the guiding role of its function orientation for medical staff and mobilize them to participate in the development of the center.