Objective: According to the participation of Shenzhen's maternal and child health care institutions in the construction of medical alliance，proposing recommendations for the development of maternal and child health care institutions. Methods: Based on field research，the symbiosis theory is applied to analyze the symbiosis relationship and experiences of the participation of maternal and child health care institutions in the construction of medical alliance in Shenzhen. Results: There are problems of unreasonable division of labour，inadequate supporting policies and poor flow of resources in medical alliance，resulting in weak linkages within the associations and a lack of mutually beneficial symbiotic relationships. Conclusion: It is recommended to establish maternal and child health care institutions-alliance，and promote the transformation of existing medical alliance to a mutually beneficial symbiosis model by optimizing the mechanism of division of labor and collaboration，improving relevant supporting policies and building a unified health information platform.