Objective: To explore the regional spatial clustering of medical service prices，so as to provide references for government to promote the regional coordination of medical service price and the homogenization of cross-region medical services. Methods: Taking outpatient consultation fees of tertiary hospitals in 41 cities in the Yangtze River Delta Region as samples，the geographic information system was used for spatial visual description，and spatial autocorrelation method was adopted to analyze the spatial clustering of medical service prices. Results: For the outpatient costs in the tertiary hospital in the Yangtze River Delta Region，Global Moran's I=0.284 \quad(Z=3.334，P=0.0008)，there was positive spatial autocorrelation. There were high-high aggregation in Jiaxing，Shaoxing in Zhejiang Province and Nantong，Suzhou in Jiangsu Province，low-low aggregation in Bengbu，Bozhou，Chizhou，Tongling and W uhu in Anhui Province，low-high aggregation in Zhoushan in Zhejiang Province (P＜0.05)，and no significant aggregation in other regions (P＞0.05). Conclusion: The spatial clustering had been found in outpatient costs in the tertiary hospital in the Yangtze River Delta Region. It was recommended to strengthen the regional coordination of medical service prices，construct rational price ratio，and promote regional health integration.