Objective: To compare the prices of medical service projects in tertiary medical institutions in Wuhan and 9 other similar cities in China, understand the pricing level of Wuhan, and provide a basis for price adjustment. Methods: Using the charging fre- quency of medical service projects in Wuhan to construct weights, calculate the weighted average price and compare the overall price level; compare comprehensive medical services, medical technology diagnosis and treatment, clinical diagnosis and treatment, traditional Chinese medicine, and traditional ethnic medicine diagnosis and treatment. Results: The current pricing of medical services in Wuhan is generally in the middle to upper range of the regions, with significantly higher than average pricing for surgical items by category and lower pricing for comprehensive medical services and medical and technical treatment items. The prices of medical services are not correlated with per capita disposable income across the regions. Conclusion: The pricing of medical service projects requires cal- culating their cost and value, and increasing monitoring of disease diagnosis group prices; establish regional medical service price monitoring indicators, such as price indices, and establish standardized information systems.